After studying flute at the Conservatory in Kroměříž, Jan Rozehnal pursued his studies in orchestral conducting at Janáček Academy of Performing Arts n Brno with Otakar Trhlík and Lubomír Mátl. His studies were further extended by a two-year internship at the Czech Philharmonic.
Already during his studies in Brno, he founded and conducted the Brno Youth Choir, with which he won several awards at international competitions. After completing his studies, he worked as an assistant to Lubomír Mátl in the Choral Society of Moravian Teachers and later became the conductor of the institution.
He started his professional career as a conductor of the South Bohemian Theater in České Budějovice. Subsequently, he was choir master of Brno Philharmonic Choir "Beseda brněnská", associated with Brno Philharmonic.
From 1990 to 2003, he was the choir master of the Slovak Philharmonic Choir. With the ensemble, he experienced many exciting moments on both domestic and international stages. During his time in Slovakia, he founded the choir Camerata Bratislava, whose domain was the historically informed performance of early music. The ensemble won the Diapason d’or and the prize of the Prime Minister of the Slovak Republic.
In 2004 Jan Rozehnal moved to Prague, where he was choir master of the Kühn Mixed Choir and the Prague Philharmonic Choir until 2008. From 2008 to 2023 he worked as choir master and conductor in Germany, first in Koblenz, Frankfurt and Halberstadt. From the 2023/2024 season Jan Rozehnal has been again the choir master of the Slovak Philharmonic Choir.
Throughout his musical career, he has been involved in various productions and recordings for renowned companies such as Decca, Deutsche Grammophon, Discovery, Harmonia Mundi, Naxos, NBC, and Slovart. He has collaborated with ensembles and conductors of great reputation, including Claudio Abbado, Riccardo Chailly, Christoph von Dohnányi, Kurt Masur, Zubin Mehta, Georges Prêtre, and Ralf Weikert.